Trypsin Inhibitor Antikörper (Biotin)

Produktnummer ABIN1607819

Produktdetails anti-Trypsin Inhibitor Antikörper (Biotin)

Target: Trypsin Inhibitor

Reaktivität: Lima bean

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Trypsin Inhibitor Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Trypsin Inhibitor from other sources is unknown.

Reinheit: Anti-Trypsin Inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Trypsin Inhibitor [Lima Bean]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Trypsin Inhibitor antibody has been assayed against 1.0 µg of Trypsin Inhibitor in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:6.000 to 1:26.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Trypsin Inhibitor

Target: Trypsin Inhibitor

Abstract: Trypsin Inhibitor Produkte

Synonyme: CRISP11 antikoerper, LCRISP2 antikoerper, GRMZM2G447785 antikoerper, cysteine rich secretory protein LCCL domain containing 2 antikoerper, trypsin inhibitor antikoerper, trophoblast Kunitz domain protein 1 antikoerper, Kazal-type serine proteinase inhibitor 1 antikoerper, Bowman-Birk type bran trypsin inhibitor antikoerper, CRISPLD2 antikoerper, LOC548021 antikoerper, TKDP1 antikoerper, Spi1 antikoerper, LOC100284348 antikoerper

Hintergrund: Synonyms: 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Gen-ID: 547831

UniProt: P01070