RNASE1 Antikörper (Biotin)

Produktnummer ABIN1607821

Produktdetails anti-RNASE1 Antikörper (Biotin)

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNASE1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Kreuzreaktivität (Details): Cross reactivity against Ribonuclease A from other sources may occur but have not been specifically determined.

Reinheit: Anti-Ribonuclease A antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Ribonuclease A [Bovine Pancreas]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Ribonuclease A antibody has been assayed against 1.0 µg of Ribonuclease A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:13.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu RNASE1

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Andere Bezeichnung: Ribonuclease A (RNASE1 Produkte)

Synonyme: RNASE1 antikoerper, RNS1 antikoerper, ATRNS1 antikoerper, RIBONUCLEASE 1 antikoerper, T17M13.16 antikoerper, T17M13_16 antikoerper, ribonuclease 1 antikoerper, RIB1 antikoerper, AI574248 antikoerper, Rib-1 antikoerper, Rib1 antikoerper, SRN antikoerper, ribonuclease A family member 1, pancreatic antikoerper, ribonuclease pancreatic-like antikoerper, ribonuclease A family member k6 antikoerper, Ribonuclease 1 antikoerper, ribonuclease 1 antikoerper, ribonuclease, RNase A family, 1 (pancreatic) antikoerper, ribonuclease pancreatic antikoerper, RNASE1 antikoerper, LOC475395 antikoerper, RNASE6 antikoerper, Rnase1 antikoerper, RNS1 antikoerper, LOC101113761 antikoerper

Hintergrund: Anti-Ribonuclease A recognizes ribonuclease, an endoribonuclease that catalyzes the cleavage of the phosphodiester bond between 5'-ribose of a nucleotide and the phosphate group of a 3'-ribose of a pyrimidine nucleotide. Able to survive acidic and near boiling conditions, RNase A is highly stable. RNase A and its homologs have been demonstrated to have cytotoxic and cytostatic effects. Synonyms: Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody

Gen-ID: 282340

UniProt: P61823