Thymic Stromal Lymphopoietin Antikörper

Produktnummer ABIN1607858

Produktdetails anti-Thymic Stromal Lymphopoietin Antikörper

Target: Thymic Stromal Lymphopoietin (TSLP)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Thymic Stromal Lymphopoietin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity with TSLP from other sources has not been determined.

Reinheit: Anti-TSLP is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human TSLP protein. A BLAST analysis was used to suggest cross-reactivity with TSLP from human sources based on 100 % homology with the immunizing sequence.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.
Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: TSLP purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to the human TSLP protein by western blotting in appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:50.000
Western Blot Dilution: 1:1000-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Andere Bezeichnung: TSLP (TSLP Produkte)

Synonyme: TSLP antikoerper, thymic stromal lymphopoietin antikoerper, TSLP antikoerper, Tslp antikoerper

Hintergrund: Thymic Stromal Lymphopoietin (TSLP) is a hematopoietic cytokine. TSLP is mainly expressed in the heart, liver and prostate. TSLP sends signals through a heterodimeric receptor complex, containing the TSLP-R and the IL-7Rα chain to induce the release of T cell-attracting chemokines from monocytes. It can induce allergic inflammation by directly activating mast cells. It also promotes T helper type 2 (TH2) cell responses associated with immunity, including atopic dermatitis and asthma, and can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+ TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of dendritic cells derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues. Recombinant human TSLP is a non-glycosylated protein with a molecular weight of 15 kDa. Anti-TSLP antibody is ideal for researchers interested in cytokines and stem cell research. Synonyms: Thymic Stromal Lymphopoietin

Gen-ID: 85480

NCBI Accession: NP_149024

UniProt: Q969D9