Thymic Stromal Lymphopoietin Antikörper (Biotin)

Produktnummer ABIN1607864

Produktdetails anti-Thymic Stromal Lymphopoietin Antikörper (Biotin)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Thymic Stromal Lymphopoietin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity with TSLP from other sources has not been determined.

Reinheit: Anti-TSLP is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human TSLP protein. A BLAST analysis was used to suggest cross-reactivity with TSLP from human sources based on 100 % homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.
Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: TSLP purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to the human TSLP protein by western blotting in appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:50.000
Fluorochrome Protein Value: 10-20
Western Blot Dilution: 1:1000-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with 100 µL deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Andere Bezeichnung: TSLP (TSLP Produkte)

Synonyme: TSLP antikoerper, thymic stromal lymphopoietin antikoerper, TSLP antikoerper, Tslp antikoerper

Hintergrund: Thymic Stromal Lymphopoietin (TSLP) is a hematopoietic cytokine. TSLP is mainly expressed in the heart, liver and prostate. TSLP sends signals through a heterodimeric receptor complex, containing the TSLP-R and the IL-7Rα chain to induce the release of T cell-attracting chemokines from monocytes. It can induce allergic inflammation by directly activating mast cells. It also promotes T helper type 2 (TH2) cell responses associated with immunity, including atopic dermatitis and asthma, and can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+ TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of dendritic cells derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues. Recombinant human TSLP is a non-glycosylated protein with a molecular weight of 15 kDa. Anti-TSLP antibody is ideal for researchers interested in cytokines and stem cell research. Synonyms: Thymic Stromal Lymphopoietin

Gen-ID: 85480

NCBI Accession: NP_149024

UniProt: Q969D9