HDAC7 Antikörper (N-Term)

Produktnummer ABIN1607889

Produktdetails anti-HDAC7 Antikörper

Target: HDAC7 (Histone Deacetylase 7 (HDAC7))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HDAC7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Kreuzreaktivität (Details): Cross reactivity with HDAC7 from other sources has not been determined.

Reinheit: Anti-HDAC7 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC7. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat and Primate based on 100 % sequence homology.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: HDAC7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7.
Immunogen Type: Peptide

Anwendungsinformationen

Applikationshinweise: Anti-HDAC7 antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~103kDa and isoforms at ~70, and 40 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 1 µg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.55 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: Expiration date is six (6) months from date of opening.

Details zu HDAC7

Target: HDAC7 (Histone Deacetylase 7 (HDAC7))

Andere Bezeichnung: HDAC7 (HDAC7 Produkte)

Synonyme: HD7A antikoerper, HDAC7A antikoerper, 5830434K02Rik antikoerper, HD7 antikoerper, HD7a antikoerper, Hdac7a antikoerper, mFLJ00062 antikoerper, hdac7a antikoerper, K2K18.5 antikoerper, K2K18_5 antikoerper, histone deacetylase7 antikoerper, histone deacetylase 7 antikoerper, histone deacetylase7 antikoerper, HDAC7 antikoerper, Hdac7 antikoerper, hdac7 antikoerper, HDA7 antikoerper

Hintergrund: HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription. Anti-HDAC7 antibodies are ideal for researches interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research. Synonyms: DKFZP586J0917, EC 3.5.1.98, HD7, HD7A, HD7a, HDAC7AFLJ99588, histone deacetylase 7, Histone deacetylase 7ADKFZp586J0917

Gen-ID: 51564

NCBI Accession: NP_001091886

UniProt: Q8WUI4

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development