SOD3 Antikörper (AA 227-236)

Produktnummer ABIN1686654

Produktdetails anti-SOD3 Antikörper

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Bindungsspezifität: AA 227-236

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: Detects extracellular SOD ~35 kDa.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Peptide Affinity Purified

Immunogen: Peptide corresponding to AA 227-236 of human EC SOD

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN1686654 was sufficient for detection of ECSOD in 20 μg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu SOD3

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Andere Bezeichnung: SOD3 (SOD3 Produkte)

Synonyme: EC-SOD antikoerper, ECSOD antikoerper, SOD3 antikoerper, AI314465 antikoerper, LOC780439 antikoerper, fb05f10 antikoerper, si:dkey-117m1.6 antikoerper, sod3 antikoerper, wu:fb05f10 antikoerper, ECSODPT antikoerper, CuZnSOD antikoerper, SODA.4 antikoerper, superoxide dismutase 3 antikoerper, superoxide dismutase 3, extracellular antikoerper, superoxide dismutase 3, extracellular b antikoerper, superoxide dismutase 1 antikoerper, superoxide dismutase precursor ,putative antikoerper, SOD3 antikoerper, Sod3 antikoerper, sod3b antikoerper, sod3 antikoerper, Sod1 antikoerper, Smp_095980 antikoerper

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Gen-ID: 6649

UniProt: P08294