ENPP3 Antikörper (FITC)

Produktnummer ABIN1690738

Produktdetails anti-ENPP3 Antikörper (FITC)

Target: ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ENPP3 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: HEK-293 cells transfected with human CD203c

Klon: NP4D6

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Kommentare:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-ENPP3 Antikörper (FITC) (ABIN1690738)

Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, (2015) (PubMed).

Platz, Binder, Marxer, Lischka, Valent, Bühring: "Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals." in: International archives of allergy and immunology, Vol. 126, Issue 4, pp. 335-42, (2002) (PubMed).

Bühring, Seiffert, Giesert, Marxer, Kanz, Valent, Sano: "The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3." in: Blood, Vol. 97, Issue 10, pp. 3303-5, (2001) (PubMed).

Bühring, Simmons, Pudney, Müller, Jarrossay, van Agthoven, Willheim, Brugger, Valent, Kanz: "The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors." in: Blood, Vol. 94, Issue 7, pp. 2343-56, (1999) (PubMed).

Details zu ENPP3

Target: ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))

Andere Bezeichnung: CD203c (ENPP3 Produkte)

Synonyme: B10 antikoerper, CD203c antikoerper, NPP3 antikoerper, PD-IBETA antikoerper, PDNP3 antikoerper, Npp3 antikoerper, RB13-6 antikoerper, AI876438 antikoerper, ENPP3 antikoerper, ectonucleotide pyrophosphatase/phosphodiesterase 3 antikoerper, ectonucleotide pyrophosphatase/phosphodiesterase 3 L homeolog antikoerper, ENPP3 antikoerper, Enpp3 antikoerper, enpp3 antikoerper, enpp3.L antikoerper

Hintergrund: Ectonucleotide pyrophosphatase/phosphodiesterase 3,CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.,ENPP3, B10, PDNP3, PD-IBETA

Gen-ID: 5169

UniProt: O14638

Pathways: Regulation of Muscle Cell Differentiation, Negative Regulation of Transporter Activity