ANAPC10 Antikörper (AA 31-130) (Biotin)

Produktnummer ABIN1700786

Produktdetails anti-ANAPC10 Antikörper (Biotin)

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Bindungsspezifität: AA 31-130

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ANAPC10 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Ratte

Homologie: Human,Mouse,Cow,Sheep,Pig,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APC10

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu ANAPC10

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Andere Bezeichnung: APC10 (ANAPC10 Produkte)

Synonyme: APC10 antikoerper, DOC1 antikoerper, 1500026N15Rik antikoerper, A830003M23Rik antikoerper, CG11419 antikoerper, Dmel\\CG11419 antikoerper, ANAPC10 antikoerper, SPBC1E8.06 antikoerper, anapc10 antikoerper, MGC85037 antikoerper, DDBDRAFT_0202332 antikoerper, DDBDRAFT_0235160 antikoerper, DDB_0202332 antikoerper, DDB_0235160 antikoerper, anaphase promoting complex subunit 10 antikoerper, Anaphase Promoting Complex subunit 10 antikoerper, anaphase promoting complex subunit DOC1 antikoerper, anaphase-promoting complex subunit Apc10 antikoerper, anaphase promoting complex subunit antikoerper, predicted protein antikoerper, anaphase promoting complex subunit 10 S homeolog antikoerper, ANAPC10 antikoerper, Anapc10 antikoerper, APC10 antikoerper, DOC1 antikoerper, apc10 antikoerper, CAALFM_C107850CA antikoerper, anapc10 antikoerper, LOC692856 antikoerper, anapc10.S antikoerper, NAEGRDRAFT_66759 antikoerper

Hintergrund:

Synonyms: ANAPC 10, anapc10, Anaphase promoting complex 10, Anaphase promoting complex subunit 10, Anaphase-promoting complex subunit 10, Apc 10, APC10, APC10_HUMAN, Cyclosome subunit 10, DKFZP564L0562, Doc 1, Doc1, OTTHUMP00000220297, OTTHUMP00000220298, OTTHUMP00000220299, OTTHUMP00000220300, OTTHUMP00000220303.

Background: Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in telophase and cytokinesis.

Gen-ID: 10393

Pathways: M Phase