ELTD1 Antikörper (AA 301-400)
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- Target Alle ELTD1 Antikörper anzeigen
- ELTD1 (EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1))
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Bindungsspezifität
- AA 301-400
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Reaktivität
- Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser ELTD1 Antikörper ist unkonjugiert
- Applikation
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Kreuzreaktivität
- Maus, Ratte
- Homologie
- Human,Horse
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human ETL/ELTD1
- Isotyp
- IgG
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- Applikationshinweise
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- by
- Memorial Sloan-Kettering Cancer Center, Parada lab
- No.
- #100001
- Datum
- 15.10.2015
- Antigen
- ELTD1
- Chargennummer
- Validierte Anwendung
- Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
- Positivkontrolle
- Tumor cell transplanted mouse brain was dissected
- Negativkontrolle
- Bewertung
- The staining pattern was as expected for ELTD1 as membrane protein.
- Primärantikörper
- ABIN1713780
- Sekundärantikörper
- Donkey-anti-Rabbit-Cy3
- Full Protocol
- Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
- Immunostaining for Cryo Sections:
- Dry slides at 50-55°C for 15-30 min (or RT for 30min)
- Wash three times with PBS 5min (shake gently)
- Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
- Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
- Rabbit-anti-ELTD1 antibody (ABIN1713780) diluted 1:50-1:400
- Wash three times with PBS 5min (shake gently)
- Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
- Donkey-anti-Rabbit-Cy3
- DAPI 1:100
- Wash three times with PBS 5min (shake gently)
- Add three drops of water-based Immu-mount
- Cover with 24x60 cover glass
- Imaging
- Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
- Anmerkungen
- ABIN1713780 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.
Validierung #100001 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))ValidierungsbilderProtokoll -
- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- ELTD1 (EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1))
- Andere Bezeichnung
- ETL/ELTD1 (ELTD1 Produkte)
- Synonyme
- ELTD1 antikoerper, ETL antikoerper, KPG_003 antikoerper, Etl antikoerper, wu:fd10a02 antikoerper, zgc:63629 antikoerper, 1110033N21Rik antikoerper, ETL1 antikoerper, adhesion G protein-coupled receptor L4 antikoerper, ADGRL4 antikoerper, Adgrl4 antikoerper, adgrl4 antikoerper
- Hintergrund
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Synonyms: ETL, ELTD1, KPG_003, Adhesion G protein-coupled receptor L4, EGF, latrophilin and seven transmembrane domain-containing protein 1, EGF-TM7-latrophilin-related protein, ETL protein, ADGRL4, UNQ202/PRO228
Background: Endothelial orphan receptor that acts as a key regulator of angiogenesis.
- Gen-ID
- 64123
- UniProt
- Q9HBW9
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