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ZBTB7A Antikörper (AA 321-420)

ZBTB7A Reaktivität: Human WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro), ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1714221
  • Target Alle ZBTB7A Antikörper anzeigen
    ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))
    Bindungsspezifität
    • 14
    • 12
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 321-420
    Reaktivität
    • 56
    • 22
    • 17
    • 10
    • 7
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    Human
    Wirt
    • 56
    • 2
    Kaninchen
    Klonalität
    • 55
    • 3
    Polyklonal
    Konjugat
    • 27
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ZBTB7A Antikörper ist unkonjugiert
    Applikation
    • 49
    • 19
    • 18
    • 13
    • 13
    • 10
    • 5
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
    Kreuzreaktivität
    Human
    Homologie
    Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Pokemon
    Isotyp
    IgG
    Top Product
    Discover our top product ZBTB7A Primärantikörper
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))
    Andere Bezeichnung
    Pokemon/ZBTB7 (ZBTB7A Produkte)
    Synonyme
    ZBTB7A antikoerper, FBI-1 antikoerper, FBI1 antikoerper, LRF antikoerper, ZBTB7 antikoerper, ZNF857A antikoerper, pokemon antikoerper, 9030619K07Rik antikoerper, 9130006G12Rik antikoerper, AI452336 antikoerper, Lrf antikoerper, Pokemon antikoerper, Zbtb7 antikoerper, OCZF antikoerper, zinc finger and BTB domain containing 7A antikoerper, zinc finger and BTB domain containing 7C antikoerper, zinc finger and BTB domain containing 7a antikoerper, ZBTB7A antikoerper, ZBTB7C antikoerper, Zbtb7a antikoerper
    Hintergrund

    Synonyms: Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, FBI1, HIV-1 1st-binding protein 1, Leukemia/lymphoma related factor, LRF, Pokemon, TIP21, TTF-I interacting peptide 21, ZBTB7, ZBTB7A, Zinc finger and BTB domain-containing protein 7A, ZBT7A_HUMAN.

    Background: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.

    Gen-ID
    51341
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