14-3-3 GRF Antikörper

Produktnummer ABIN1720824

Der Kaninchen Polyklonal anti-14-3-3 GRF Antikörper (ABIN1720824) detektiert spezifisch 14-3-3 GRF in IP und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Green bean, Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum, Neochloris oleoabundans, Oryza sativa, Panax notoginseng, Picrorhiza kurroa, Vicia faba und Mais (Zea mays).

Kurzübersicht für 14-3-3 GRF Antikörper (ABIN1720824)

Target: 14-3-3 GRF

Reaktivität: Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Green bean, Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum, Neochloris oleoabundans, Oryza sativa, Panax notoginseng, Picrorhiza kurroa, Vicia faba, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-14-3-3 GRF | General regulatory element

Spezifität: TAIR: At4g09000, At4g78300, At4g38480, At4g35160, At4g16050, At4g10450, At4g02520, At4g65430, At4g42590, At4g22300, At4g34760

Kreuzreaktivität (Details): Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Lilium longiflorum, Mesembryanthemum crystallinum, Neochloris oloabundans (algae), Oryza sativa, Picrorhiza kurroa, Phaseolus vulgaris, Panax notoginseng, Zea mays, Vicia faba
Not reactive in: This antibody does not bind to 14-3-3-like protein D of Nicotiana tabacum.

Homologie: Brassica sp.,Chlamydomonas reinhardtii,Glycine max,Gossypium hirsutum,Nicotiana tabacum,Physcomitrium patens,Pisum sativum,Solanum tuberosum,Triticum aestivum

Aufreinigung: Serum

Immunogen: Conserved surface-exposed peptide conjugated to KLH.This sequence is repeated in all 12 ""canonical"" members of the Arabidopsis thaliana GRF/14-3-3 family. GRF1 GF14 chi UniProt: F4JJ94

Anwendungsinformationen

Applikationshinweise: 1: 2000 (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: 14-3-3 GRF

Hintergrund:

Background: GRF (General regulatory factor 2) or 14.3.3s are 30 kDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway.

Additional information: This antibody is recognizing recombiant GRF of Lilium longiflorum Lil1433_0 accession: AF191746, Lil1433_2, accession: EF397608 and recombinant GRF1,2,3,5 and 6 of Arabidopsis thaliana: GRF1 14-3-3 chi (At4g09000.1), GRF2 14-3-3 omega (At1g78300.1), GRF3 14-3-3 psi (At5g38480.1), GRF5 14-3-3 upsilon (At5g16050.1), GRF6 14-3-3 lambda (At5g10450.2), GRF8 14-3-3 kappa (At5g65430.1), GRF11 14-3-3 omicron (At1g34760.1).There is also very weak reaction to Physcomitrella patens Pp14-3-3 Pp1s 73_133V6 (closest homolog to AtGRF6) and Chlamydomonas reinhardtii Cr 14-3-3 Cre 12.g559250 (closest homolog to AtGRF6).

Molekulargewicht: 20-28 kDa (depending upon an isoform)

Gen-ID: 818859, 831462

UniProt: F4JJ94, Q01525, F4KBI7, F4HWQ5, P42645, Q96300, F4KHY7, F4IP55, F4I1C1, Q9S9Z8, Q9C5W6