HRP Antikörper

Produktnummer ABIN1724681

Produktdetails anti-HRP Antikörper

Target: HRP (Horseradish Peroxidase (HRP))

Reaktivität: Meerrettich

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HRP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Immunogen: Purified recombinant fragment of HRP expressed in E. Coli.

Klon: 3A5C6

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C or at -20 °C for long term.

Referenzen für anti-HRP Antikörper (ABIN1724681)

Villegas: "Time course of angiogenesis in solid pineal autografts." in: Cell and tissue research, Vol. 315, Issue 3, pp. 349-59, (2004) (PubMed).

Metelitza, Karasyova, Grintsevich, Thorneley: "Peroxidase-catalyzed co-oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of substituted phenols and their polydisulfides." in: Journal of inorganic biochemistry, Vol. 98, Issue 1, pp. 1-9, (2003) (PubMed).

Details zu HRP

Target: HRP (Horseradish Peroxidase (HRP))

Andere Bezeichnung: HRP (HRP Produkte)

Hintergrund: Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.