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CD22 Antikörper (AA 621-725)

Name: CD22 Antikörper (AA 621-725)
Brand: antibodies-online
SKU: ABIN1724888
Price: 614.71 EUR
Availability: InStock

CD22 Reaktivität: Human FACS, WB, ELISA, IHC, ICC Wirt: Maus Monoclonal 2H1C4 unconjugated

Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red). (CD22 Antikörper (AA 621-725))

FACS

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (CD22 Antikörper (AA 621-725))

ELISA

Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). (CD22 Antikörper (AA 621-725))

Western blot analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A432 (6) and NIH/3T3 (7) cell lysate. (CD22 Antikörper (AA 621-725))

Western blot analysis using CD22 mAb against human CD22 recombinant protein. (CD22 Antikörper (AA 621-725))

Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate. (CD22 Antikörper (AA 621-725))

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining. (CD22 Antikörper (AA 621-725))

IHC

Immunohistochemical analysis of paraffin-embedded cerebellum tissues using ZEB1 mouse mAb with DAB staining. (CD22 Antikörper (AA 621-725))

IHC

8 Abbildungen

(2 Referenzen)

Produktnummer ABIN1724888

Datenblatt (PDF)

Target Alle CD22 Antikörper anzeigen

CD22 (CD22 Molecule (CD22))
Bindungsspezifität
16

11

7

5

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 621-725
Reaktivität
143

52

24

5

3

2

2

1
Human
Wirt
90

62

16
Maus
Passende Sekundärantikörper
Klonalität
115

55
Monoklonal
Konjugat
83

15

13

11

8

4

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Dieser CD22 Antikörper ist unkonjugiert
Applikation
102

72

44

38

35

20

12

12

11

8

5

4

3

3

3

2

2

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Verwendungszweck

CD22 Antibody

Aufreinigung

Purified antibody

Immunogen

Purified recombinant fragment of human CD22 (AA: 621-725) expressed in E. Coli.

Klon

2H1C4

Isotyp

IgG1
anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 Reaktivität: Human FACS, ELISA, ICC Wirt: Maus Monoclonal 1A3A11 unconjugated

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 Reaktivität: Human FACS, WB, ELISA, IHC, IF, Coat, StM Wirt: Maus Monoclonal BLCAM-1795 unconjugated

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 Reaktivität: Human FACS, WB, ELISA, IHC, IF, Coat Wirt: Maus Monoclonal BLCAM-1796 unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 Reaktivität: Human, Non-Human Primate FACS, WB, IP Wirt: Maus Monoclonal MEM-01 unconjugated

anti-CD22 Molecule (CD22) antibody (PE)
CD22 Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal MEM-01 PE

anti-CD22 Molecule (CD22) antibody (FITC)
CD22 Reaktivität: Human FACS Wirt: Maus Monoclonal IS7 FITC

anti-CD22 Molecule (CD22) (AA 708-847) antibody
CD22 Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 Reaktivität: Human, Maus, Ratte, Affe, Hund WB, IHC, IHC (p) Wirt: Maus Monoclonal 4C3 unconjugated

anti-CD22 Molecule (CD22) (AA 20-687) antibody
CD22 Reaktivität: Human FACS, WB, ELISA, IF, IP Wirt: Kaninchen Monoclonal DM13 unconjugated

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 Reaktivität: Human IHC Wirt: Kaninchen Monoclonal MSVA-022R unconjugated Recombinant Antibody

Applikationshinweise

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50 - 1/200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Tuscano, Kato, Pearson, Xiong, Newell, Ma, Gandara, ODonnell: "CD22 antigen is broadly expressed on lung cancer cells and is a target for antibody-based therapy." in: Cancer research, Vol. 72, Issue 21, pp. 5556-65, (2012) (PubMed).

Kawasaki, Rademacher, Paulson: "CD22 regulates adaptive and innate immune responses of B cells." in: Journal of innate immunity, Vol. 3, Issue 4, pp. 411-9, (2011) (PubMed).
Target

CD22 (CD22 Molecule (CD22))

Andere Bezeichnung

CD22 (CD22 Produkte)

Synonyme

A530093D23 antikoerper, Lyb-8 antikoerper, Lyb8 antikoerper, SIGLEC-2 antikoerper, SIGLEC2 antikoerper, siglec-2 antikoerper, CD22 antigen antikoerper, CD22 molecule antikoerper, Cd22 antikoerper, CD22 antikoerper

Hintergrund

Description: CD22 may be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins, one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules
Aliases: SIGLEC2, SIGLEC-2

Molekulargewicht

95.3kDa

Gen-ID

933

HGNC

933

UniProt

P20273