WB: 1 : 1000 up to 1 : 5000 (AP staining) ICC: 1 : 1000 IHC: 1 : 500
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
PBS
Handhabung
Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C. Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
van Stegen, Dagar, Gottmann: "Release activity-dependent control of vesicle endocytosis by the synaptic adhesion molecule N-cadherin." in: Scientific reports, Vol. 7, pp. 40865, (2017) (PubMed).
Schaefer, Toral, Velez, Cox, Baker, Borcherding, Colgan, Bondada, Mashburn, Yu, Geddes, Tsang, Bassuk, Mahajan: "Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2509-21, (2016) (PubMed).
Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).
Target
VAMP2
(Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))