SNAP alpha/beta Antikörper

Produktnummer ABIN1742242

Produktdetails anti-SNAP alpha/beta Antikörper

Target: SNAP alpha/beta

Reaktivität: Human, Ratte, Maus, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SNAP alpha/beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Spezifität: Specific for alpha- and beta SNAP

Kreuzreaktivität (Details): does not cross-react to gamma SNAP.

Aufreinigung: purified IgG

Immunogen: Recombinant full length rat alpha SNAP.

Klon: 77-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: not tested yet

Kommentare:

The antibody does not immunoprecipitate the 20 S SNARE-complex.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-SNAP alpha/beta Antikörper (ABIN1742242)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

Details zu SNAP alpha/beta

Target: SNAP alpha/beta

Abstract: SNAP alpha/beta Produkte