Neuroligin 1/2/3/4 Antikörper (AA 1-695)

Produktnummer ABIN1742285

Produktdetails anti-Neuroligin 1/2/3/4 Antikörper

Target: Neuroligin 1/2/3/4 (NLGN1/2/3/4)

Bindungsspezifität: AA 1-695

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunoprecipitation (IP), ELISA, Western Blotting (WB)

Spezifität: Recognizes rat and mouse neuroligins 1-3 and rat neuroligin 4.

Aufreinigung: purified IgG

Immunogen: Recombinant protein of rat neuroligin 1 containing the extracellular sequence (aa 1-695).

Klon: 4F9

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
ICC: not recommended
IHC: not recommended

Kommentare:

ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-Neuroligin 1/2/3/4 Antikörper (ABIN1742285)

Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

Fujita-Jimbo, Tanabe, Yu, Kojima, Mori, Li, Iwamoto, Yamagata, Momoi, Momoi: "The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis." in: Molecular autism, Vol. 6, pp. 17, (2015) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Bottos, Destro, Rissone, Graziano, Cordara, Assenzio, Cera, Mascia, Bussolino, Arese: "The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 49, pp. 20782-7, (2010) (PubMed).

De Jaco, Comoletti, Kovarik, Gaietta, Radic, Lockridge, Ellisman, Taylor: "A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9667-76, (2006) (PubMed).

Chih, Engelman, Scheiffele: "Control of excitatory and inhibitory synapse formation by neuroligins." in: Science (New York, N.Y.), Vol. 307, Issue 5713, pp. 1324-8, (2005) (PubMed).

Details zu Neuroligin 1/2/3/4

Target: Neuroligin 1/2/3/4 (NLGN1/2/3/4)

Andere Bezeichnung: Neuroligin