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GFP Antikörper

Name: GFP Antikörper
SKU: ABIN1742293
Price: 481.38 EUR
Availability: InStock

GFP Reaktivität: Aequorea victoria WB, ICC Wirt: Kaninchen Polyclonal unconjugated

dilution: 1 : 1000, sample: total cell lysate of GFP transfected fibroblasts (GFP Antikörper)

Image no. 1 for anti-Green Fluorescent Protein (GFP) antibody (ABIN1742293) (GFP Antikörper)

2 Abbildungen

(1 reference)

Produktnummer ABIN1742293

Datenblatt (PDF)

Target Alle GFP Antikörper anzeigen

GFP (Green Fluorescent Protein (GFP))
Reaktivität
178

15

11

9
Aequorea victoria
Wirt
64

63

34

13

9

5

4

2
Kaninchen
Passende Sekundärantikörper
Klonalität
113

76
Polyklonal
Konjugat
83

12

11

10

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GFP Antikörper ist unkonjugiert
Applikation
148

80

48

34

28

27

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), Immunocytochemistry (ICC)

Kreuzreaktivität (Details)

Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

Aufreinigung

Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen

Strep-Tag® fusion protein, full-length GFP.

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Applikationshinweise

WB: 1 : 1000 up to 1 : 20000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet

Kommentare

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer

PBS

Handhabung

Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung

-20 °C

Informationen zur Lagerung

Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Rotmann, Strand, Martiné, Closs: "Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54185-92, (2004) (PubMed).
Target

GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung

GFP (GFP Produkte)

Synonyme

green fluorescent protein antikoerper, gfp antikoerper