GFP Antikörper (CypHer5E)
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- Target Alle GFP Antikörper anzeigen
- GFP (Green Fluorescent Protein (GFP))
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Reaktivität
- Aequorea victoria
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser GFP Antikörper ist konjugiert mit CypHer5E
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Applikation
- Immunocytochemistry (ICC)
- Kreuzreaktivität (Details)
- Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).
- Aufreinigung
- Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization.
- Immunogen
- Strep-Tag® fusion protein, full-length GFP.
- Top Product
- Discover our top product GFP Primärantikörper
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- Applikationshinweise
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IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet - Kommentare
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ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
- Buffer
- PBS
- Handhabung
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Lagerung
- -20 °C
- Informationen zur Lagerung
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Fluorescence-labeled antibodies should be reconstituted immediately upon delivery.
Prolonged storage at 4 °C is not recommended!
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How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).
: "
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How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).
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- Target
- GFP (Green Fluorescent Protein (GFP))
- Andere Bezeichnung
- GFP (GFP Produkte)
- Synonyme
- green fluorescent protein antikoerper, gfp antikoerper
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