Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 596-705) Antikörper

Produktnummer ABIN1742305

Produktdetails

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Bindungsspezifität: AA 596-705

Reaktivität: Maus, Ratte, Human, Huhn, Frosch, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Spezifität: Specific for RIM 1

Kreuzreaktivität (Details): weak cross reactivity to RIM 2.

Aufreinigung: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein (aa 596-705) of rat RIM 1 containing part of PDZ domain.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 100 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Referenzen

Tang, Chen, Li, Metzbower, MacGillavry, Blanpied: "A trans-synaptic nanocolumn aligns neurotransmitter release to receptors." in: Nature, Vol. 536, Issue 7615, pp. 210-4, (2016) (PubMed).

Grabner, Gandini, Rehak, Le, Zamponi, Schmitz: "RIM1/2-Mediated Facilitation of Cav1.4 Channel Opening Is Required for Ca2+-Stimulated Release in Mouse Rod Photoreceptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 38, pp. 13133-47, (2015) (PubMed).

Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

Ramírez-Franco, Bartolomé-Martín, Alonso, Torres, Sánchez-Prieto: "Cannabinoid type 1 receptors transiently silence glutamatergic nerve terminals of cultured cerebellar granule cells." in: PLoS ONE, Vol. 9, Issue 2, pp. e88594, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." in: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

Bello, Zanetti, Mayorga, Michaut: "RIM, Munc13, and Rab3A interplay in acrosomal exocytosis." in: Experimental cell research, Vol. 318, Issue 5, pp. 478-88, (2012) (PubMed).

Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, (2011) (PubMed).

Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Regus-Leidig, Tom Dieck, Specht, Meyer, Brandstätter: "Early steps in the assembly of photoreceptor ribbon synapses in the mouse retina: the involvement of precursor spheres." in: The Journal of comparative neurology, Vol. 512, Issue 6, pp. 814-24, (2008) (PubMed).

Jüttner, Moré, Das, Babich, Meier, Henning, Erdmann, Mu Ller, Otto, Grantyn, Rathjen: "Impaired synapse function during postnatal development in the absence of CALEB, an EGF-like protein processed by neuronal activity." in: Neuron, Vol. 46, Issue 2, pp. 233-45, (2005) (PubMed).

Antigendetails

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Andere Bezeichnung: RIM 1 (RIMS1 Produkte)

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity