ERC2
Reaktivität: Human
WB, IF (p), IF (cc)
Wirt: Kaninchen
Polyclonal
AbBy Fluor® 647
Applikationshinweise
WB: 1 : 100 up to 1 : 2000 (AP staining) IP: not tested yet ICC: not recommended IHC: not recommended
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in TBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handhabung
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Lagerung
-20 °C
Informationen zur Lagerung
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).
Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).
tom Dieck, Altrock, Kessels, Qualmann, Regus, Brauner, Fejtová, Bracko, Gundelfinger, Brandstätter: "Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex." in: The Journal of cell biology, Vol. 168, Issue 5, pp. 825-36, (2005) (PubMed).
Target
ERC2
(ELKS/RAB6-Interacting/CAST Family Member 2 (ERC2))