CACNA1B Antikörper (AA 1921-2222)
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- Target Alle CACNA1B Antikörper anzeigen
- CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))
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Bindungsspezifität
- AA 1921-2222
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Reaktivität
- Ratte, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser CACNA1B Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC)
- Spezifität
- Specific for Ca2+ channel alpha-1E.
- Aufreinigung
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Recombinant protein (aa 1921-2222 of rat Ca2+-channel alpha-1E subunit).
- Top Product
- Discover our top product CACNA1B Primärantikörper
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- Applikationshinweise
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WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 - Kommentare
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WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: IHC: This antibody requires a mild fixation protocol (1 % PFA in sodium acetate buffer, pH 6).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handhabung
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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- Target
- CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))
- Andere Bezeichnung
- Ca2+ Channel (CACNA1B Produkte)
- Synonyme
- BIII antikoerper, CACNL1A5 antikoerper, CACNN antikoerper, Cav2.2 antikoerper, AW050276 antikoerper, AW060892 antikoerper, AW822256 antikoerper, Cchn1a antikoerper, alpha(1B) antikoerper, calcium voltage-gated channel subunit alpha1 B antikoerper, calcium channel, voltage-dependent, N type, alpha 1B subunit antikoerper, CACNA1B antikoerper, Cacna1b antikoerper
- Hintergrund
- Synonyms: Cav2.3
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