Homer 1/2/3 (N-Term) Antikörper
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- Target
- Homer 1/2/3
- Bindungsspezifität
- N-Term
- Reaktivität
- Human, Maus, Ratte, Zebrafisch (Danio rerio)
- Wirt
- Kaninchen
- Klonalität
- Polyklonal
- Applikation
- Immunocytochemistry (ICC), Western Blotting (WB)
- Spezifität
- The first 120 aa were used for affinity purification. According to Soloviev et al. (2000), they are homologous in Homer 1 a-d, Homer 2 a-d, and Homer 3 a-d.
- Aufreinigung
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Purified recombinant N-terminal parts of Homer 1, 2 and 3.
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- Applikationshinweise
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet
ELISA: not tested yet - Kommentare
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ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handhabung
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).
: "Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear." in: Biological psychiatry, Vol. 68, Issue 11, pp. 1007-15, (2010) (PubMed).
: "
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Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).
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- Target
- Homer 1/2/3
- Hintergrund
- Synonyms: VesL 1, Syn47
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