Abeta-pE3 Antikörper

Produktnummer ABIN1742433

Produktdetails

Target: Abeta-pE3

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Spezifität: Specific for Abeta-pE3.

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide pyr-EFRHD coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. Epitope Minimal epitope: pyr-EFR.

Klon: 1-57

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000
ICC: not tested yet
IHC: 1 : 100

Kommentare:

WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen

Antonios, Saiepour, Bouter, Richard, Paetau, Verkkoniemi-Ahola, Lannfelt, Ingelsson, Kovacs, Pillot, Wirths, Bayer: "N-truncated Abeta starting with position four: early intraneuronal accumulation and rescue of toxicity using NT4X-167, a novel monoclonal antibody." in: Acta neuropathologica communications, Vol. 1, Issue 1, pp. 56, (2013) (PubMed).

Wittnam, Portelius, Zetterberg, Gustavsson, Schilling, Koch, Demuth, Blennow, Wirths, Bayer: "Pyroglutamate amyloid β (Aβ) aggravates behavioral deficits in transgenic amyloid mouse model for Alzheimer disease." in: The Journal of biological chemistry, Vol. 287, Issue 11, pp. 8154-62, (2012) (PubMed).

Antigendetails

Target: Abeta-pE3

Hintergrund: Synonyms: pyro-Glu Abeta, pE-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42)