Abeta-pE3 Antikörper
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- Target
- Abeta-pE3
- Reaktivität
- Human, Maus, Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Unkonjugiert
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Applikation
- Western Blotting (WB), Immunocytochemistry (ICC)
- Spezifität
- Recognizes specific oligomeric structures formed preferentially by Abeta-pE3.
- Aufreinigung
- purified IgG. Azide was added before lyophilization.
- Immunogen
- Synthetic peptide pyr-EFRHDSGYEVHHQK coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. Epitope Minimal epitope: pyr-EFRHDS.
- Klon
- 70D7
- Isotyp
- IgG3
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- Applikationshinweise
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WB: 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100
ELISA: not tested yet - Kommentare
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WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS, 0.02% sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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- Target
- Abeta-pE3
- Hintergrund
- Synonyms: pyro-Glu Abeta, pE-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42)
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