Abeta 38/40/42 (AA 1-16), (AA 4-8) Antikörper

Produktnummer ABIN1742439

Produktdetails

Target: Abeta 38/40/42

Bindungsspezifität: AA 1-16, AA 4-8

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Specific for Abeta 38, 40, 42.

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide (aa 1-16) of abeta peptides 38, 40, 42 coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. Epitope aa 4-8

Klon: NT244

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC-P: 1 : 500
ELISA: not tested yet

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen

Porcellotti, Fanelli, Fracassi, Sepe, Cecconi, Bernardi, Cimini, Cerù, Moreno: "Oxidative Stress during the Progression of β-Amyloid Pathology in the Neocortex of the Tg2576 Mouse Model of Alzheimer's Disease." in: Oxidative medicine and cellular longevity, Vol. 2015, pp. 967203, (2015) (PubMed).

Reinert, Martens, Huettenrauch, Kolbow, Lannfelt, Ingelsson, Paetau, Verkkoniemi-Ahola, Bayer, Wirths: "Aβ38 in the brains of patients with sporadic and familial Alzheimer's disease and transgenic mouse models." in: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 4, pp. 871-81, (2014) (PubMed).

Wirths, Hillmann, Pradier, Härtig, Bayer: "Oligomeric pyroglutamate amyloid-β is present in microglia and a subfraction of vessels in patients with Alzheimer's disease: implications for immunotherapy." in: Journal of Alzheimer's disease : JAD, Vol. 35, Issue 4, pp. 741-9, (2013) (PubMed).

Venkataramani, Wirths, Budka, Härtig, Kovacs, Bayer: "Antibody 9D5 recognizes oligomeric pyroglutamate amyloid-β in a fraction of amyloid-β deposits in Alzheimer's disease without cross-reactivity with other protein aggregates." in: Journal of Alzheimer's disease : JAD, Vol. 29, Issue 2, pp. 361-71, (2012) (PubMed).

Antigendetails

Target: Abeta 38/40/42