WB: 1 : 1000 IP: not tested yet ICC: 1 : 200 up to 1 : 500 IHC: 1 : 100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handhabung
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Lagerung
-20 °C
Informationen zur Lagerung
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Körber, Horstmann, Venkataramani, Herrmannsdörfer, Kremer, Kaiser, Schwenger, Ahmed, Dean, Dresbach, Kuner: "Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover." in: Neuron, Vol. 87, Issue 3, pp. 521-33, (2015) (PubMed).
Mendoza Schulz, Jing, Sánchez Caro, Wetzel, Dresbach, Strenzke, Wichmann, Moser: "Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse." in: The EMBO journal, Vol. 33, Issue 5, pp. 512-27, (2014) (PubMed).
Target
TPRG1L
(Tumor Protein P63 Regulated 1-Like (TPRG1L))