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beta Actin Antikörper (AA 2-16)

ACTB Reaktivität: Maus, Ratte, Huhn, Zebrafisch (Danio rerio) WB, IHC, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1742508
  • Target Alle beta Actin (ACTB) Antikörper anzeigen
    beta Actin (ACTB) (Actin, beta (ACTB))
    Bindungsspezifität
    • 39
    • 16
    • 15
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-16
    Reaktivität
    • 177
    • 126
    • 107
    • 56
    • 43
    • 41
    • 37
    • 25
    • 23
    • 22
    • 18
    • 17
    • 12
    • 10
    • 9
    • 7
    • 7
    • 6
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus, Ratte, Huhn, Zebrafisch (Danio rerio)
    Wirt
    • 112
    • 79
    • 5
    • 1
    • 1
    Kaninchen
    Klonalität
    • 110
    • 86
    Polyklonal
    Konjugat
    • 124
    • 12
    • 12
    • 10
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser beta Actin Antikörper ist unkonjugiert
    Applikation
    • 164
    • 70
    • 50
    • 46
    • 35
    • 30
    • 26
    • 22
    • 13
    • 13
    • 8
    • 6
    • 6
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Kreuzreaktivität (Details)
    May cross-react to alpha- and gamma-actin due to sequence homology.
    Aufreinigung
    Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
    Immunogen
    Synthetic peptide (aa 2-16 of mouse beta-actin) coupled to KLH via a C-terminally added cysteine.
    Top Product
    Discover our top product ACTB Primärantikörper
  • Applikationshinweise
    WB: 1 : 1000 up to 1 : 5000
    IP: not tested yet
    ICC: 1 : 200 up to 1 : 500
    IHC: 1 : 200
    Kommentare

    ICC: Methanol fixation is recommended.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029836 (Western Blotting)
    'Independent Validation' Siegel
    by
    Kinexus Bioinformatics Corporation
    No.
    #029836
    Datum
    22.06.2015
    Antigen
    Chargennummer
    251003/3
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    mouse brain, mouse kidney, mouse muscle, mouse spleen, mouse testes
    Negativkontrolle
    none [ubiquitously expressed]
    Bewertung
    A band was observed in the positive controls at the expected size (~42 kDa)
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: Beta-Actin, (ACTB) (AA 2-16)
    • Catalog number: ABIN1742553
    • Lot number: 251003/3
    • Dilution: 1:1000
    Sekundärantikörper
    • Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
    • Lot number: F0613
    • Dilution: 1:10,000
    Full Protocol
    • 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
    • 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
    • 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
    • 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
    • 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
    • 6. The membrane was washed in TTBS twice for 5 minutes each.
    • 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
    • 8. The membrane was rinsed twice with TTBS.
    • 9. The membrane was washed in TTBS twice for 5 minutes each.
    • 10. The membrane was washed in TTBS once for 15 minutes.
    • 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
    • 12. The membrane was rinsed twice with TTBS.
    • 13. The membrane was washed in TTBS twice for 5 minutes each.
    • 14. The membrane was washed in TTBS once for 15 minutes.
    • 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 160 seconds.
    • 16. The membrane was rinsed three times with TTBS.
    • 17. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
    • 18. The membrane was washed in TTBS 3 times for 5 minutes each.
    • 19. Repeated Steps 4-14 with the loading control antibody (for β3−Tubulin).
    • 20. Repeated Steps 4-14 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
    Anmerkungen
    A protein band was observed at the expected target size of β−Actin of 42 kDa in all mouse extracts tested. Lower molecular weight bands of approximately 28-30 kDa were also observed in the mouse kidney and spleen tissue samples. Three main groups of actin have been identified. The β-actins and γ-actins co-exist in most cell types as components of the cytoskeleton, whereas the α-actin are found in muscle tissues. In our experience, we find that the level of actin does vary widely between different cell and tissue types.
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Handhabung
    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
  • Lu, Ma, Zhuang, Zhang, Cheng, Shi: "Retinoic acid‑incorporated glycol chitosan nanoparticles inhibit the expression of Ezh2 in U118 and U138 human glioma cells." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 6642-8, (2016) (PubMed).

    Baron, Blex, Rohrbeck, Rachakonda, Birnbaumer, Ahnert-Hilger, Brunk: "The α-subunit of the trimeric GTPase Go2 regulates axonal growth." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 782-94, (2013) (PubMed).

    Whitemarsh, Strathman, Chase, Stankewicz, Tepp, Johnson, Pellett: "Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 126, Issue 2, pp. 426-35, (2012) (PubMed).

  • Target
    beta Actin (ACTB) (Actin, beta (ACTB))
    Andere Bezeichnung
    beta-Actin (ACTB Produkte)
    Pathways
    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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