Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
Spezifität
Recognizes GalphaO 1 and GalphaO 2
Aufreinigung
purified IgG
Immunogen
Recombinant full length rodent GalphaO 2.
Klon
101-1
Isotyp
IgG2b
Applikationshinweise
WB: 1 : 1000 (AP staining) ICC: 1 : 500 up to 1 : 1000 IHC: not tested yet
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handhabung
Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Lagerung
-20 °C
Informationen zur Lagerung
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Baron, Blex, Rohrbeck, Rachakonda, Birnbaumer, Ahnert-Hilger, Brunk: "The α-subunit of the trimeric GTPase Go2 regulates axonal growth." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 782-94, (2013) (PubMed).
Winter, Brunk, Walther, Höltje, Jiang, Peter, Takamori, Jahn, Birnbaumer, Ahnert-Hilger: "Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 18, pp. 4672-80, (2005) (PubMed).