Tubulin delta 2 (AA 443-449) Antikörper

Produktnummer ABIN1742551

Produktdetails

Target: Tubulin delta 2

Bindungsspezifität: AA 443-449

Reaktivität: Human, Säugetier, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Spezifität: Specific for delta2-tubulin.

Aufreinigung: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet

Kommentare:

This antibody has been cross-adsorbed against peptides corresponding to the C-termini of glu- and tyr-tubulin. ICC: Requires long permeabilization with 0.3% Tritonx100 for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Antigendetails

Target: Tubulin delta 2