NCK1 Antikörper

Produktnummer ABIN1774727

Produktdetails anti-NCK1 Antikörper

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NCK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Spezifität: The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.

Kreuzreaktivität (Details): Human, Mouse

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Purified recombinant second SH3 domain of human Nck1 fused to GST

Klon: EM-06

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 2 μg/mL, positive control: Jurkat cell lysate.
Immunocytochemistry: Recommended dilution: 2 μg/mL, positive control: human NCK1-transfected COS-7 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze. Do not use after expiration date stamped on vial label.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-NCK1 Antikörper (ABIN1774727)

Borroto, Arellano, Dopfer, Prouza, Suchànek, Fuentes, Orfao, Schamel, Alarcón: "Nck recruitment to the TCR required for ZAP70 activation during thymic development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 3, pp. 1103-12, (2013) (PubMed).

Details zu NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Andere Bezeichnung: NCK1 (NCK1 Produkte)

Hintergrund: NCK adaptor protein 1,NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.,NCK-1, NCKalpha

Gen-ID: 4690

UniProt: P16333

Pathways: T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals