Soluble Terminal Complement Complex (sC5b-9) Antikörper

Produktnummer ABIN181111

Produktdetails

Target: Soluble Terminal Complement Complex (sC5b-9)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktmerkmale: Synonyms: Terminal complement complex, Membrane attack complex

Aufreinigung: Affinity Chromatography on Protein A

Klon: 3R2-0

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot. Flow Cytometry. Immunohistochemistry on frozen sections. Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Borate buffered saline, pH 8.4 containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Antigendetails

Target: Soluble Terminal Complement Complex (sC5b-9)

Andere Bezeichnung: Complement SC5b-9 (TCC / MAC)

Hintergrund: Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kDa C5b which is bound to cells via 71 kDa C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kDa S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation.Synonyms: Membrane attack complex, Terminal complement complex