CXCL14 Antikörper
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- Target Alle CXCL14 Antikörper anzeigen
- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser CXCL14 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Spezifität
- This antibody detects BRAK.
- Aufreinigung
- Immunoaffinity Chromatography using an immobilized Human BRAK matrix
- Immunogen
- Highly pure (> 98%) E.coli derived recombinant Human BRAK
- Top Product
- Discover our top product CXCL14 Primärantikörper
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- Applikationshinweise
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ELISA: Indirect: To detect hBRAK (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK. Sandwich: To detect hBRAK by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human BRAK as a detection antibody, it allows the detection of at least0.2 - 0.4 ng/well of recombinant hBRAK. Western blot: To detect hBRAK this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBRAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
- Haltbarkeit
- 6 months
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- Target
- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
- Andere Bezeichnung
- CXCL14 / BRAK (CXCL14 Produkte)
- Hintergrund
- BRAK/CXCL14 is a CXC chemokine constitutively expressed at the mRNA level in certain normal tissues but absent from many established tumor cell lines and human cancers. Although multiple investigators have cloned BRAK, little is known regarding the physiologic function of BRAK or the reason for decreased expression in cancer.Synonyms: C-X-C motif chemokine 14, NJAC, SCYB14, Small-inducible cytokine B14
- Gen-ID
- 9547
- NCBI Accession
- NP_004878
- UniProt
- O95715
- Pathways
- Autophagie
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