ELISA: Direct: To detect hTECK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK. Sandwich: To detect hTECK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanTECK as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTECK. Western blot: To detect hTECK this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
Buffer
PBS, pH 7.2
Handhabung
This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!