This antibody SM1540A recognises natural and recombinant IL-2 expressed in either E.coli or CHO cells. Clone 4F12 neutralises the proliferative activity of rchIL-2 and recognises a different epitope to Clone 10E7 product code (Cat.-No SM1539A). This antibody can be used as the Neutralising antibody for recombinant Chicken IL-2 bioassays (See Product code SA028).
Aufreinigung
Affinity Chromatography on Protein G
Immunogen
Recombinant Chicken IL-2. Remarks: Spleen cells from immunised mice were fused with cells of the mouse Sp2/0-Ag myeloma cell line.
IL2
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: 5-10g as capture antibody. Western Blot: Non-reducing conditions. Functional Assays: 2-20g/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS without preservatives, None
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF