CD68 Antikörper (Biotin)

Produktnummer ABIN181833

Produktdetails anti-CD68 Antikörper (Biotin)

Target: CD68 (CD68 Molecule (CD68))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD68 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Spezifität: This antibody does detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.

Aufreinigung: Affinity chromatography on Protein G

Klon: FA-11

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL,Membrane permeabilisation is required for this application, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD68 Antikörper (Biotin) (ABIN181833)

Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).

Details zu CD68

Target: CD68 (CD68 Molecule (CD68))

Andere Bezeichnung: CD68 (CD68 Produkte)

Hintergrund: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.Synonyms: Gp110, Macrophage marker, Macrosialin

Gen-ID: 12514

NCBI Accession: NP_033983

UniProt: P31996