This antibody recognises the C5a receptor (C5aR) CD88. Clone P12/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31). Clone P12/1 does not inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.
Aufreinigung
Affinity Chromatography on Protein G
Immunogen
C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
C5AR1
Reaktivität: Human
WB, PLA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western Blot. Immunohistochemistry on frozen section. Immunohistochemistry on paraffin section. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
C5a Anaphylatoxin Receptor mediates the effects of one of the most potent chemotactic agents known, in a wide variety of inflammatory cell types. C5a receptor 1 has been implicated in asthma. CD88 is predominantly expressed on cells of the myeloid lineage.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1