Notch1 Antikörper
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- Target Alle Notch1 (NOTCH1) Antikörper anzeigen
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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Reaktivität
- Human, Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Notch1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Spezifität
- This antibody is specific for Notch1, a single transmembrane receptor which is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow.
- Aufreinigung
- Affinity Chromatography on Protein G
- Immunogen
- Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Klon
- MN1A
- Isotyp
- IgG1
- Top Product
- Discover our top product NOTCH1 Primärantikörper
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- Applikationshinweise
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Western Blot. Flow Cytometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections (1/50-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservataive.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
- Andere Bezeichnung
- NOTCH1 (NOTCH1 Produkte)
- Synonyme
- notch1 antikoerper, NOTCH1 antikoerper, hn1 antikoerper, tan1 antikoerper, notch antikoerper, xotch antikoerper, xnotch antikoerper, notch-1 antikoerper, xnotch1 antikoerper, TAN1 antikoerper, hN1 antikoerper, 9930111A19Rik antikoerper, Mis6 antikoerper, N1 antikoerper, Tan1 antikoerper, lin-12 antikoerper, NOTCH antikoerper, Xotch antikoerper, notch1-a antikoerper, notch 1 antikoerper, fibropellin-1 antikoerper, Notch homolog 1, translocation-associated (Drosophila) antikoerper, Notch homolog 1, translocation-associated antikoerper, notch 1 S homeolog antikoerper, notch1 antikoerper, LOC100631526 antikoerper, NOTCH1 antikoerper, Notch1 antikoerper, notch1.S antikoerper
- Hintergrund
- The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoieisis, myelopoiesis, and neurogenesis.Synonyms: Neurogenic locus notch homolog protein 1, Notch 1, TAN1, hN1
- Gen-ID
- 4851
- NCBI Accession
- NP_060087
- UniProt
- P46531
- Pathways
- Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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