MAPKAP Kinase 2 Antikörper (AA 44-55)

Produktnummer ABIN1833999

Produktdetails anti-MAPKAP Kinase 2 Antikörper

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Bindungsspezifität: AA 44-55

Reaktivität: Human, Maus, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MAPKAP Kinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Human MAPKAPK2 / MK2. This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-PQFHVKSGLQIK from an internal region of human MAPKAPK2 / MK2 (NP_004750.1, NP_116584.2). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey (100%), Galago, Mouse, Rat, Panda, Rabbit, Pig, Guinea pig (92%), Bovine, Opossum (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:32000), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 μg/mL. Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 μg EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Andere Bezeichnung: MAPKAPK2 / MK2 (MAPKAPK2 Produkte)

Synonyme: mapkapk2 antikoerper, wu:fb30e10 antikoerper, wu:fc56c10 antikoerper, wu:fi03a01 antikoerper, MGC78852 antikoerper, MAPKAPK2 antikoerper, AA960234 antikoerper, MAPKAP-K2 antikoerper, MK-2 antikoerper, MK2 antikoerper, Rps6kc1 antikoerper, mitogen-activated protein kinase-activated protein kinase 2a antikoerper, mitogen-activated protein kinase-activated protein kinase 2 L homeolog antikoerper, mitogen-activated protein kinase-activated protein kinase 2 antikoerper, MAP kinase-activated protein kinase 2 antikoerper, mapkapk2a antikoerper, mapkapk2.L antikoerper, MAPKAPK2 antikoerper, Mapkapk2 antikoerper

Hintergrund: Name/Gene ID: MAPKAPK2
Subfamily: MAPKAPK
Family: Protein Kinase

Synonyms: MAPKAPK2, MK-2, MAP KAP Kinase 2, MAPKAP kinase 2, MK2, MAPKAP-K2, MAPKAPK-2

Gen-ID: 9261

NCBI Accession: NP_004750, NP_116584

UniProt: P49137

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades