PRKAA1/PRKAA2 Antikörper (pThr172, pThr174)

Produktnummer ABIN1842476

Produktdetails anti-PRKAA1/PRKAA2 Antikörper

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Bindungsspezifität: pThr172, pThr174

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAA1/PRKAA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Spezifität: AMPKalpha,1/AMPKalpha,2 Antibody detects endogenous levels of total AMPKalpha,1/AMPKalpha,2 protein.

Produktmerkmale: Molecular Weight: Predicted Band Size 63 kDa, Observed Band Size 63 kDa

Aufreinigung: Rabbit Anti-AMPKalpha,1/AMPKalpha,2 Antibody (Ab-174/172) Polyclonal Antibody was purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from human AMPKalpha,1/AMPKalpha,2 around pThr174,pThr172 (L-R-TP-S-C).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC: 1:50-1:100,
WB: 1:500-1:1,000,
IF: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for 1 year.

Referenzen für anti-PRKAA1/PRKAA2 Antikörper (ABIN1842476)

Arduini, Serviddio, Escobar, Tormos, Bellanti, Viña, Monsalve, Sastre: "Mitochondrial biogenesis fails in secondary biliary cirrhosis in rats leading to mitochondrial DNA depletion and deletions." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 301, Issue 1, pp. G119-27, (2011) (PubMed).

Details zu PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Abstract: PRKAA1/PRKAA2 Produkte

Synonyme: AMPK antikoerper, AMPK2 antikoerper, AMPKa2 antikoerper, PRKAA antikoerper, Ampk antikoerper, Ampka2 antikoerper, 2310008I11Rik antikoerper, A830082D05 antikoerper, AMPKalpha2 antikoerper, protein kinase AMP-activated catalytic subunit alpha 2 antikoerper, protein kinase, AMP-activated, alpha 2 catalytic subunit antikoerper, PRKAA2 antikoerper, Prkaa2 antikoerper

Hintergrund: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-COA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-COA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.