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Strep Tag II Antikörper (HRP)

WB, ELISA Wirt: Kaninchen Polyclonal HRP
Produktnummer ABIN1842500
  • Target
    Strep Tag II
    Reaktivität
    Bitte anfragen
    Wirt
    • 9
    • 3
    Kaninchen
    Klonalität
    • 9
    • 3
    Polyklonal
    Konjugat
    • 7
    • 2
    • 2
    • 1
    HRP
    Applikation
    • 11
    • 7
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA
    Sequenz
    NWSHPQFEK
    Spezifität
    This Antibody recognizes Strep-tag II tagged fusion proteins.
    Aufreinigung
    Immunoaffinity
    Immunogen
    Strep-tag II epitope peptide NWSHPQFEK conjugated to KLH.
    Isotyp
    IgG
  • Applikationshinweise
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0. 05-0. 2 µg/mL
    Western blot: 0. 5-1. 0 µg/mL
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
    Konzentration
    0.5 mg/mL
    Buffer
    lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
    Konservierungsmittel
    Thimerosal (Merthiolate)
    Vorsichtsmaßnahmen
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
  • Target
    Strep Tag II
    Substanzklasse
    Tag
    Hintergrund
    Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered. The Rabbit anti NWSHPQFEK polyclonal antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain NWSHPQFEK.
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