Rabbit Anti-Smad2 (Phospho-Ser467) Polyclonal Antibody detects endogenous levels of Smad2 only when phosphorylated at serine 467.
Produktmerkmale
Molecular Weight: Predicted Band Size 60 kDa, Observed Band Size 60 kDa
Aufreinigung
Rabbit Anti-Smad2 (Phospho-Ser467) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Synthesized phosphopeptide derived from human Smad2 around pSer467 (C-S-S-M-SP)
SMAD2
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
WB: 1:500-1:1,000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1 mg/mL
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage., Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store Rabbit Anti-Smad2 (Phospho-Ser467) Polyclonal Antibody at -20 °C. It will remain stable for one year.
Song, Thalacker, Nilsen-Hamilton: "Synergistic and multidimensional regulation of plasminogen activator inhibitor type 1 expression by transforming growth factor type ? and epidermal growth factor." in: The Journal of biological chemistry, Vol. 287, Issue 15, pp. 12520-8, (2012) (PubMed).
Target
SMAD2
(SMAD, Mothers Against DPP Homolog 2 (SMAD2))