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CBP Tag Antikörper

WB, ELISA Wirt: Maus Monoclonal 1D11E5 unconjugated
Produktnummer ABIN1842584
  • Target Alle CBP Tag Produkte
    CBP Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 16
    • 7
    Maus
    Klonalität
    • 16
    • 6
    Monoklonal
    Konjugat
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CBP Tag Antikörper ist unkonjugiert
    Applikation
    • 13
    • 13
    • 9
    • 8
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA
    Sequenz
    KRRWKKNFIA VSAA
    Spezifität
    CBP Tag Antibody, mAb, Mouse recognizes CBP-tagged proteins.
    Produktmerkmale
    CBP Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with CBP tag peptide conjugated to KLH.
    Aufreinigung
    Protein A affinity column
    Immunogen
    CBP tag peptide conjugated to KLH
    Klon
    1D11E5
    Isotyp
    IgG2b kappa
  • Applikationshinweise
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    Western blot: 1 µg/mL.
    Immunoprecipitation: 1-5 µg/mL.
    ELISA: 0. 001-0. 002 µg/mL.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
    Konzentration
    0.5 mg/mL
    Buffer
    lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
  • Li, Liu, Zhang, Issaian, Hill, Espinosa, Shi, Cui, Kappel, Das, Hansen, Zhou, Zhao: "A unified mechanism for intron and exon definition and back-splicing." in: Nature, Vol. 573, Issue 7774, pp. 375-380, (2020) (PubMed).

  • Target
    CBP Tag
    Abstract
    CBP Tag Produkte
    Substanzklasse
    Tag
    Hintergrund
    CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins
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