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SIAH1 Antikörper (N-Term)

Name: SIAH1 Antikörper (N-Term)
Brand: antibodies-online
SKU: ABIN184675
Price: 792.57 EUR
Availability: InStock

Verified SIAH1 Reaktivität: Human, Ratte WB, ELISA, IF Wirt: Ziege Polyclonal unconjugated

ABIN184675 staining (0. (SIAH1 Antikörper (N-Term))

(ABIN184675) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (SIAH1 Antikörper (N-Term))

ABIN184675 (1µg/ml) staining of Mouse (A, C) and Rat (B, D) Brain (A, B) and Liver (C, D) lysates (35µg protein in RIPA buffer). (SIAH1 Antikörper (N-Term))

ABIN184675 staining (1µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). (SIAH1 Antikörper (N-Term))

(ABIN184675) (1 μg/mL) staining of Human Liver lysate (35 μg protein in RIPA buffer). (SIAH1 Antikörper (N-Term))

(ABIN184675) (1 μg/mL) staining of RatLiver lysate (35 μg protein in RIPA buffer). (SIAH1 Antikörper (N-Term))

(ABIN184675) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. (SIAH1 Antikörper (N-Term))

8 Abbildungen

(1 reference)

Produktnummer ABIN184675

Datenblatt (PDF)

Target Alle SIAH1 Antikörper anzeigen

SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
Bindungsspezifität
15

7

6

2

2

2

2

1

1

1

1

1
N-Term
Reaktivität
39

32

32

7

7

6

4

4

4

3

3

2

2

2

2

1
Human, Ratte
Wirt
51

2

1
Ziege
Passende Sekundärantikörper
Klonalität
52

2
Polyklonal
Konjugat
21

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser SIAH1 Antikörper ist unkonjugiert
Applikation
39

17

13

13

7

5

3

3

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Verwendungszweck

SIAH1

Sequenz

SRQTATALPT GTSKC

Spezifität

This product is expected to recognize both reported isoforms (NP_003022.3, NP_001006611.1)

Kreuzreaktivität

Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Immunogen

Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3, NP_001006611.1.

Isotyp

IgG
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibody
SIAH1 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibody
SIAH1 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 2C5 unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody
SIAH1 Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Zebrafisch (Danio rerio), Pferd, Meerschweinchen, Kaninchen WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody
SIAH1 Reaktivität: Human, Ratte, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 191-240) antibody
SIAH1 Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Zebrafisch (Danio rerio), Pferd, Meerschweinchen, Kaninchen, Xenopus laevis, Huhn, Schwein, Fledermaus, Hamster, Affe WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (C-Term) antibody
SIAH1 Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Zebrafisch (Danio rerio), Pferd, Meerschweinchen, Kaninchen WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibody
SIAH1 Reaktivität: Human ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 201-282) antibody
SIAH1 Reaktivität: Ratte, Maus WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibody
SIAH1 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 2F26 unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody
SIAH1 Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Zebrafisch (Danio rerio), Pferd, Meerschweinchen, Xenopus laevis, Huhn, Schwein, Fledermaus, Hamster, Affe WB Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Western Blot: Approx. 37 kDa band observed in Human Liver lysates and approx. 30+37 kDa bands in Rat Liver lysates (calculated MW of 34.6 kDa according to Human NP_001006611.1 and 31.1 kDa according to Rat NP_543181.2 and 38.2 kDa according to Rat XP_03895336
Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10μg/ml.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Sroka, Voigt, Deeg, Reed, Schulz, Bhr, Kermer: "BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." in: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).
Target

SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Andere Bezeichnung

SIAH1 (SIAH1 Produkte)

Hintergrund

SIAH1, seven in absentia homolog 1 (Drosophila), Siah-1, hSIAH1, HUMSIAH, Siah-1a, FLJ08065, seven in absentia homolog 1, sonic hedgehog homolog

Molekulargewicht

31.1kDa

Gen-ID

6477, 20437, 140941

NCBI Accession

NP_003022, NP_001006611