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FOXP3 Antikörper (C-Term)

Name: FOXP3 Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN184677
Price: 792.57 EUR
Availability: InStock

Verified FOXP3 Reaktivität: Human WB, FACS, ELISA, IHC, IF, ICC Wirt: Ziege Polyclonal unconjugated

ABIN184677 (1µg/ml) staining of Human Muscle (A), (2ug/ml) MOLT4 (B) and (1µg/ml) negative control Pancreas (C) lysate (35µg protein in RIPA buffer). (FOXP3 Antikörper (C-Term))

ABIN184677 (1µg/ml) staining of paraffin embedded Human Tonsil. (FOXP3 Antikörper (C-Term))

IHC

ABIN184677 staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B). (FOXP3 Antikörper (C-Term))

ICC

ABIN184677 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (FOXP3 Antikörper (C-Term))

ABIN184677 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0. (FOXP3 Antikörper (C-Term))

FACS

5 Abbildungen

Produktnummer ABIN184677

Datenblatt (PDF)

Target Alle FOXP3 Antikörper anzeigen

FOXP3 (Forkhead Box P3 (FOXP3))
Bindungsspezifität
16

15

11

11

6

6

6

5

5

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reaktivität
143

124

50

26

6

3

2

2

1

1

1

1

1

1

1

1
Human
Wirt
96

67

20

1
Ziege
Passende Sekundärantikörper
Klonalität
96

88
Polyklonal
Konjugat
89

15

11

10

7

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
Dieser FOXP3 Antikörper ist unkonjugiert
Applikation
112

87

54

39

33

27

27

17

16

15

8

7

7

6

4

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Verwendungszweck

FOXP3 / SCURFIN

Sequenz

SQRPSRCSNP TPGP

Spezifität

This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

Kreuzreaktivität

Human

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Immunogen

Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2, NP_001107849.1.

Isotyp

IgG

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Applikationshinweise

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx 48-50 kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2 kDa according to NP_054728.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negat
Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

FOXP3 (Forkhead Box P3 (FOXP3))

Andere Bezeichnung

FOXP3 (FOXP3 Produkte)

Hintergrund

FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin

Gen-ID

50943

NCBI Accession

NP_054728, NP_001107849

Pathways

Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation