RACGAP1 Antikörper (C-Term)

Produktnummer ABIN184690

Produktdetails anti-RACGAP1 Antikörper

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser RACGAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: RACGAP1 / MgcRacGAP

Sequenz: GRQGNFFASP MLK

Spezifität: This antibody is expected to recognize all reported isoforms (NP_037409.2, NP_001306934.1, NP_001306935.1, NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_0011

Kreuzreaktivität: Rind (Kuh), Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0 kDa according to NP_037409.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room te

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.<

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu RACGAP1

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Andere Bezeichnung: RACGAP1 (RACGAP1 Produkte)

Synonyme: CYK4 antikoerper, HsCYK-4 antikoerper, ID-GAP antikoerper, MgcRacGAP antikoerper, cyk-4 antikoerper, id-gap antikoerper, mgcracgap antikoerper, RACGAP1 antikoerper, MGC121578 antikoerper, Gap antikoerper, RasGAP antikoerper, Rasa antikoerper, GAPX antikoerper, AI227039 antikoerper, AI327394 antikoerper, Band25 antikoerper, GTPase antikoerper, gtl11 antikoerper, mKIAA1478 antikoerper, wu:fb92c08 antikoerper, zgc:77839 antikoerper, Rac GTPase activating protein 1 antikoerper, rac GTPase-activating protein 1 antikoerper, Rac GTPase activating protein 1 L homeolog antikoerper, RAS p21 protein activator 1 antikoerper, Rac GTPase-activating protein 1 antikoerper, si:ch1073-416j23.1 antikoerper, RACGAP1 antikoerper, racgap1 antikoerper, racgap1.L antikoerper, racgap1.2.L antikoerper, racgap1.2 antikoerper, Rasa1 antikoerper, Racgap1 antikoerper, si:ch1073-416j23.1 antikoerper

Hintergrund: RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4

Gen-ID: 29127

NCBI Accession: NP_037409, NP_001306934, NP_001306935, NP_001306936

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor