PLK4 Antikörper (N-Term)
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- Target Alle PLK4 Antikörper anzeigen
- PLK4 (Polo-Like Kinase 4 (PLK4))
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Bindungsspezifität
- N-Term
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser PLK4 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- Sak / STK18 / PLK4
- Sequenz
- ATCIGEKIED FKVGN
- Homologie
- Human, Dog, Cow
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence ATCIGEKIEDFKVGN, from the N Terminus of the protein sequence according to NP_055079.3.
- Isotyp
- IgG
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- Applikationshinweise
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DS WB Results: Approx 110 kDa band observed in Human Colon lysates (calculated MW of 109 kDa according to NP_055079.3). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- PLK4 (Polo-Like Kinase 4 (PLK4))
- Andere Bezeichnung
- PLK4 (PLK4 Produkte)
- Synonyme
- CG7186 antikoerper, DmSAK antikoerper, Dmel\\CG7186 antikoerper, PLK4 antikoerper, Plk4 antikoerper, SAK/PLK antikoerper, SAK/PLK4 antikoerper, Sak antikoerper, plk4 antikoerper, sak antikoerper, SAK antikoerper, STK18 antikoerper, 1700028H20 antikoerper, AI385771 antikoerper, Stk18 antikoerper, PLK-4 antikoerper, stk18 antikoerper, wu:fi33g07 antikoerper, Sak kinase antikoerper, polo like kinase 4 antikoerper, polo-like kinase 4 antikoerper, polo-like kinase 4 (Drosophila) antikoerper, serine/threonine-protein kinase PLK4 antikoerper, SAK antikoerper, PLK4 antikoerper, Plk4 antikoerper, plk4 antikoerper, LOC5571876 antikoerper
- Substanzklasse
- Phage Protein
- Hintergrund
- PLK4, Sak, polo-like kinase 4 (Drosophila), serine/threonine kinase 18, SAK, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase, STK18, serine/threonine protein kinase SAK, MCCRP2, STK 18
- Gen-ID
- 10733
- NCBI Accession
- NP_055079, NP_001177728
- Pathways
- M Phase
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