Flotillin 1 Antikörper (C-Term)

Produktnummer ABIN184756

Produktdetails anti-Flotillin 1 Antikörper

Target: Flotillin 1 (FLOT1)

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Flotillin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Verwendungszweck: Flotillin 1

Sequenz: SISQVNHKPL RTA

Spezifität: This antibody is expected to recognize both reported isoforms (NP_005794.1, NP_001305804.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: C-SISQVNHKPLRTA

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell line K562 and in preliminary testing of cell lysate MCF7 (calculated MW of 47.4 kDa according to NP_005794.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperatu

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Flotillin 1 Antikörper (ABIN184756)

Vassilieva, Gerner-Smidt, Ivanov, Nusrat: "Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G965-76, (2008) (PubMed).

Details zu Flotillin 1

Target: Flotillin 1 (FLOT1)

Andere Bezeichnung: FLOT1 (FLOT1 Produkte)

Synonyme: reggie-2 antikoerper, flot1 antikoerper, Rareg antikoerper, flot1c antikoerper, flotillin-1 antikoerper, flotillin1 antikoerper, flot1a antikoerper, reggie2 antikoerper, si:dz44o19.1 antikoerper, wu:fj45g11 antikoerper, flotillin 1 antikoerper, flotillin-1 antikoerper, putative flotillin-1 antikoerper, flotillin 1 S homeolog antikoerper, flotillin 1 L homeolog antikoerper, flotillin 1a antikoerper, FLOT1 antikoerper, Flot1 antikoerper, flot1 antikoerper, LOC5566722 antikoerper, CpipJ_CPIJ008623 antikoerper, Smp_016200.1 antikoerper, flot1.S antikoerper, flot1.L antikoerper, flot1a antikoerper

Hintergrund: FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae, DADB-118P11.2

Gen-ID: 10211, 14251

NCBI Accession: NP_005794, NP_001305804