KIF5B Antikörper (C-Term)

Produktnummer ABIN184774

Produktdetails anti-KIF5B Antikörper

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KIF5B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Verwendungszweck: Kinesin 1 / UKHC

Sequenz: QPVAVRGGGG KQV

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Immunofluorescence:This antibody has been successfully used in IF on Human: Connell et al. (20

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-KIF5B Antikörper (ABIN184774)

Connell, Allison, Rodger, Pearson, Zlamalova, Reid: "ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." in: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).

Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

Yasuda, Clatterbuck-Soper, Jackrel, Shorter, Mili: "FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination." in: The Journal of cell biology, Vol. 216, Issue 4, pp. 1015-1034, (2017) (PubMed).

Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, (2010) (PubMed).

Gupta, Palmer, Spence, Hudson, Stephens: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, (2008) (PubMed).

Details zu KIF5B

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Andere Bezeichnung: KIF5B (KIF5B Produkte)

Synonyme: KINH antikoerper, KNS antikoerper, KNS1 antikoerper, UKHC antikoerper, Khc antikoerper, AL022807 antikoerper, Khcs antikoerper, Kns1 antikoerper, Ukhc antikoerper, khc antikoerper, KIF5A antikoerper, kinesin family member 5B antikoerper, kinesin heavy chain antikoerper, kinesin-1 antikoerper, kinesin-1 heavy chain antikoerper, kinesin family member 5B S homeolog antikoerper, KIF5B antikoerper, LOC411769 antikoerper, cgd6_2790 antikoerper, kin-1 antikoerper, Tb927.1.1350 antikoerper, LACBIDRAFT_305999 antikoerper, Khc antikoerper, LOC100538629 antikoerper, Kif5b antikoerper, kif5b.S antikoerper

Hintergrund: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain

Gen-ID: 3799

NCBI Accession: NP_004512

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization