JUP Antikörper (C-Term)
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- Target Alle JUP Antikörper anzeigen
- JUP (Junction Plakoglobin (JUP))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser JUP Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Verwendungszweck
- Plakoglobin / Gamma-catenin
- Sequenz
- GLRPPYPTAD HMLA
- Spezifität
- This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.
- Kreuzreaktivität
- Hund, Human, Maus, Schwein, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.
- Isotyp
- IgG
- Top Product
- Discover our top product JUP Primärantikörper
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- Applikationshinweise
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 80 kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7 kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Kommentare
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- JUP (Junction Plakoglobin (JUP))
- Andere Bezeichnung
- JUP (JUP Produkte)
- Hintergrund
- JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80 kDa
- Gen-ID
- 3728, 16480
- NCBI Accession
- NP_002221, NP_068831
- Pathways
- Cell-Cell Junction Organization, Maintenance of Protein Location
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