UBE2C Antikörper (C-Term)
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- Target Alle UBE2C Antikörper anzeigen
- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser UBE2C Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)
- Verwendungszweck
- UBE2C / UBCH10
- Sequenz
- QETYSKQVTS QEP
- Spezifität
- This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
- Kreuzreaktivität
- Human
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1.
- Isotyp
- IgG
- Top Product
- Discover our top product UBE2C Primärantikörper
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- Applikationshinweise
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Western Blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7 kDa according to NP_008950.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
- Andere Bezeichnung
- UBE2C (UBE2C Produkte)
- Synonyme
- LOC458287 antikoerper, UBCH10 antikoerper, dJ447F3.2 antikoerper, 1110015A16Rik antikoerper, D2Ertd695e antikoerper, im:7137135 antikoerper, zgc:123190 antikoerper, ubiquitin conjugating enzyme E2 C antikoerper, ubiquitin-conjugating enzyme E2C antikoerper, ubiquitin conjugating enzyme E2 C L homeolog antikoerper, ubiquitin conjugating enzyme E2 C S homeolog antikoerper, UBE2C antikoerper, NAEGRDRAFT_82946 antikoerper, ube2c.L antikoerper, Ube2c antikoerper, ube2c.S antikoerper, ube2c antikoerper
- Hintergrund
- UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1
- Gen-ID
- 11065
- NCBI Accession
- NP_008950, NP_861515, NP_861516, NP_861517, NP_861518, NP_001268670
- Pathways
- Ubiquitin Proteasome Pathway
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